13C NMR and cerebral biochemistry.

Progress towards in vivo brain 13C-MRS in mice: Metabolic flux analysis in small tissue volumes.

The combination of dynamic 13C MRS data under infusion of 13C-labelled substrates and compartmental models of cerebral metabolism enabled in vivo measurement of metabolic fluxes with a quantitative and distinct determination of cellular-specific activities. The non-invasive nature and the chemical specificity of the 13C dynamic data obtained in those tracer experiments makes it an attractive ap...

13C isotopomer analysis of glutamate by J-resolved heteronuclear single quantum coherence spectroscopy.

13C NMR isotopomer analysis is a powerful method for measuring metabolic fluxes through pathways intersecting in the tricarboxylic acid cycle. However, the inherent insensitivity of 13C NMR spectroscopy makes application of isotopomer analysis to small tissue samples (mouse tissue, human biopsies, or cells grown in tissue culture) problematic. (1)H NMR is intrinsically more sensitive than 13C N...

Solid-state nuclear magnetic resonance evidence for an extended beta strand conformation of the membrane-bound HIV-1 fusion peptide.

Solid-state nuclear magnetic resonance (NMR) spectroscopy was applied to the membrane-bound form of a synthetic peptide representing the 23-residue N-terminal fusion peptide domain of the HIV-1 gp41 envelope glycoprotein. 1D solid-state NMR line width measurements of singly 13C carbonyl labeled peptides showed that a significant population of the membrane-bound peptide is well-structured in its...

Resolution-enhanced 4D 15N/13C NOESY protein NMR spectroscopy by application of the covariance transform.

15N/13C NOESY Protein NMR Spectroscopy by Application of the Covariance Transform David A. Snyder,† Yingqi Xu,‡ Daiwen Yang,‡ and Rafael Brüschweiler*,† Department of Chemistry and Biochemistry & National High Magnetic Field Laboratory, Florida State UniVersity, Tallahassee, Florida 32306, and Department of Biological Sciences, National UniVersity of Singapore, 14 Science DriVe 4, Singapore 117543

Natural-abundance 13C-NMR spectroscopy of two glyco-asparagines derived from the core of N-glycosidic carbohydrate chains.